ASMS Extended Abstracts and Posters:  2004, 2003, 2002, 2001, 2000, 1999, 1998

2004

Y. Yao, L. Huang, A. Krutchinsky, M-L Wong, K.G. Standing, A.L. Burlingame, and C.C Wang, "The Unique Proteasome-Activating Protein from Trypanosoma Brucei , PA26, Activates also Mammalian Proteasome" submitted to Molecular and Cellular Biology.

K.G. Standing and W. Ens, "Time-of-Flight in Mass Spectrometers", in Encyclopedia of Spectroscopy and Spectrometry , Academic Press, London, UK, in press.

I.V. Chernushevich, W. Ens, and K.G. Standing, "Orthogonal-Injection Time-of-Flight Mass Spectrometry for Analysis of Biomolecules", Anal. Chem. (A-page review), in press.

A.N. Krutchinsky, A. Ayed, L.J. Donald, W. Ens, H.W. Duckworth, and K.G. Standing, "Studies of Noncovalent Complexes in an Electrospray Ionization/Time-of-Flight Mass Spectrometer" in Methods in Molecular Biology Vol. 146 -- Peptide and Protein Analysis: Advances in the Use of Mass Spectrometry , Ed. J. Chapman, Humana Press, in press.

D.L. Seifers, T.L. Harvey, S. Haber, I. Chernushevich, W. Ens, Y-M She, and K. Standing, "Natural Infection of Sorghum by Foxtail Mosaic Virus in Kansas, Plant Disease , in press.

E. A. MacGregor, S. L. Bazin, W. Ens, J. Lahnstein, L.J. Macri, N. Shirley and A. W. MacGregor, "Structure of Barley Limit Dextrinase Inhibitors", J. Cereal Science, in press

X. Meng, J. Krishnan, Y. She, W. Ens, K. Standing, and J.A. Wilkins,  “The Association of rasGAPSH3 Binding Protein 1, G3BP1, and rasGAP120 with Integrin Containing Complexes Induced by an Adhesion Blocking Antibody”. J. Proteome Research., in press.

K. Dasuri, M. Antonovici, K. Cheng, K. Wong, K. Standing, W. Ens, H. El Gabalawy, and J. Wilkins, “The Synovial Proteome: Analysis of Fibroblast-like Synoviocytes”. Arthritis Research & Therapy, in press.

D.L. Seifers, Y-M. She, T.L Harvey, T.J. Martin, S. Haber, W. Ens, K.G. Standing K.G., R. Louie, and D.T. Gordon, " Biological and Molecular Variability Among High Plains Virus Isolates", Plant Disease, in press.

L. J. Donald, H. W. Duckworth, and K. G. Standing, “Mass Spectrometry in Non-covalent Protein Interactions and Protein Assemblies” in Cell Biology: a laboratory handbook, 3rd edition (Ed. J. Celis), Elsevier, in press.

W. Ens and K.G. Standing, “Hybrid Quadrupole/Time of Flight Mass Spectrometers for Analysis of Biomolecules” in Biological Mass Spectrometry (Ed. A.M. Burlingame), a new volume in the series Methods in Enzymology, Academic Press, in press.

Y-M. She, D.L. Seifers, S. Haber, W. Ens, and K.G. Standing, “Characterization of the Agent of “High Plains Disease: Mass Spectrometry Determines the Sequence of the Disease-specific Protein”. J. Biol. Chem. 279, 488-494 (2004).

2003

O. Krokhin, Y. Li, A. Andonov, H. Feldmann, R. Flick, S. Jones, U. Stroeher, N. Bastien, K.V. Dasuri, K. Cheng, J.N. Simonsen, H. Perreault, J. Wilkins, W. Ens, F. Plummer and K.G. Standing, "Mass Spectrometric Characterization of Proteins from the SARS Virus: A Preliminary Report.", Mol. Cell. Proteomics 2, 346 (2003).

T. Weiler, P. Sauder, K. Cheng, W. Ens, K. Standing and J. A. Wilkins, “A Proteomics-based Approach for Monoclonal Antibody Characterization”, Anal. Biochem. 321, 217-225 (2003).

K. G. Standing “Peptide and Protein De Novo Sequencing by Mass Spectrometry”, Current Opinion in Structural Biology 13, 595-601 (2003).

O. V. Krokhin, W. Ens, and K. G. Standing “Characterizing Degradation Products of Peptides Containing N-Terminal Cys Residues by (Off-Line HPLC)/MALDI-QqTOF Measurements”, Rapid Commun. Mass Spectrom 17, 2528-2534 (2003).

M. Ethier, J.A. Saba, M. Spearman, O. Krokhin, M. Butler, W. Ens, K.G. Standing and H. Perreault, “Application of the StrOligo algorithm for the Automated Sequence Assignment of Complex N-Linked Glycans from Glycoproteins Using Tandem Mass Spectrometry”, Rapid Commun. Mass Spectrom. 17, 2713-2720 (2003).

O. Krokhin, K. Cheng, S. Sousa, W. Ens, K.G. Standing & J.A. Wilkins, “Mass Spectrometric Based Mapping of the Disulphide Bonding Patterns of Integrin a Chains.”, Biochemistry 42, 12950-12959 (2003).

2002

M. Ethier, J.A. Saba, W. Ens, K.G. Standing and H. Perreault, "Automated Structural Assignment of Derivatized Complex N-linked Oligosaccharides From Tandem Mass Spectra.", Rapid Commun. Mass Spectrom. 16, 1743 (2002).

L.H. Fu, X.F. Wang, Y. Eyal, Y.M. She, L.J. Donald, K.G. Standing and G. Ben-Hayyim, "A Selenoprotein in the Plant Kingdom. Mass Spectrometry Confirms That an Opal Codon (UGA) Encodes Selenocysteine in Chlamydomonas reinhardtii Glutathione Peroxidase.", J. Biol. Chem. 277, 25983 (2002).

J.A. Saba, J.P. Kunkel, D.C. Jan, W.E. Ens, K.G. Standing, M. Butler, J.C. Jamieson and H. Perreault, "A Study of Immunoglobulin G Glycosylation in Monoclonal and Polyclonal Species by Electrospray and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.", Anal. Biochem. 305, 16 (2002).

Y.M. She, G.Q. Wang, A. Loboda, W. Ens, K.G. Standing and F.J. Burczynski, "Sequencing of Rat Liver Cytosolic Proteins by Matrix-Assisted Laser Desorption Ionization-Quadrupole Time-of-Flight Mass Spectrometry Following Electrophoretic Separation and Extraction.", Anal. Biochem. 310, 137 (2002).

2001

C.H. Lee, M.E. McComb, M. Bromirski, A. Jilkine, W. Ens, K.G. Standing and H. Perreault, "On-Membrane Digestion of Beta-Casein for Determination of Phosphorylation Sites by MALDI Quadrupole/Time-of-Flight Mass Spectrometry.", Rapid Commun. Mass Spectrom. 15, 191 (2001).

A. Shevchenko, A. Loboda, W. Ens, B. Schraven, K.G. Standing and A. Shevchenko, "Archived Polyacrylamide Gels as a Resource for Proteome Characterization by Mass Spectrometry.", Electrophoresis 22, 1194 (2001).

P. Verhaert, S. Uttenweiler-Joseph, M. de Vries, A. Loboda, W. Ens and K.G. Standing, "MALDI Quadrupole Time-of-Flight Mass Spectrometry: An Elegant Tool for Peptidomics.", Proteomics 1, 118 (2001).

L.J. Donald, D.J. Hosfield, S.L. Cuvelier, W. Ens, K.G. Standing and H.W. Duckworth, "Mass Spectrometric Study of the Escherichia Coli Repressor Proteins, Ic1R and Gc1R, and Their Complexes With DNA.", Protein Sci 10, 1370 (2001).

Y.M. She, S. Haber, D.L. Seifers, A. Loboda, I. Chernushevich, H. Perreault, W. Ens and K.G. Standing, "Determination of the Complete Amino Acid Sequence for the Coat Protein of Brome Mosaic Virus by Time-of-Flight Mass Spectrometry.", J. Biol. Chem. 276, 20039 (2001).

T.J. Griffin, S.P. Gygi, B. Rist, R. Aebersold, A. Loboda, A. Jilkine, W. Ens and K.G. Standing, "Quantitative Proteomic Analysis Using a MALDI Quadrupole Time-of-Flight Mass Spectrometer.", Anal. Chem. 73, 978 (2001).

A. Shevchenko, S. Sunyaev, A. Loboda, A. Shevchenko, P. Bork, W. Ens and K.G. Standing, "Charting the Proteomes of Organisms With Unsequenced Genomes by MALDI-Quadrupole Time-of-Flight Mass Spectrometry and BLAST Homology Searching.", Anal. Chem. 73, 1917 (2001).

M.E. McComb, R.D. Oleschuk, A. Chow, R.G. Dworschak, M. Znamirowski, K.R. Preston, W. Ens, K.G. Standing, and H. Perreault, "Application of Non-Porous Polyurethane (PU) Thin Films and Porous PU Membranes As Sample Supports for MALDI-MS of Wheat Proteins", Can. J. Chem. 79 437 (2001).

2000

A.V. Loboda, A.N. Krutchinsky, M. Bromirski, W. Ens and K.G. Standing, "A Tandem Quadrupole/Time-of-Flight Mass Spectrometer With a Matrix-Assisted Laser Desorption/Ionization Source: Design and Performance", Rapid Commun. Mass Spectrom. 14, 1047 (2000).

A.N. Krutchinsky, A. Ayed, L.J. Donald, W. Ens, H.W. Duckworth and K.G. Standing, "Studies of Noncovalent Complexes in an Electrospray Ionization/Time-of-Flight Mass Spectrometer.", Methods Mol. Biol. 146, 239 (2000).

A. Shevchenko, A. Loboda, A. Shevchenko, W. Ens and K.G. Standing, "MALDI Quadrupole Time-of-Flight Mass Spectrometry: A Powerful Tool for Proteomic Research.", Anal. Chem. 72, 2132 (2000).

A. Loboda, A. Krutchinsky, O. Loboda, J. McNabb, V. Spicer, W. Ens, and K.G. Standing, "Novel Linac II Electrode Geometry for Creating an Axial Field in a Multipole Ion Guide", Eur. J. Mass Spectrom. 6 531 (2000).

1999

A.N. Krutchinsky, I.V. Chernushevich, A.V. Loboda, W. Ens, and K.G. Standing, "Measurements of Protein Structure and Noncovalent Interactions by Time-of-Flight Mass Spectrometry with Orthogonal Ion Injection", in Proc. 4th Int. Symposium on Mass Spectrometry in the Health and Life Sciences , San Francisco CA 25-29 Aug. 1998, Humana Press, in press.

Z Zhang, A Krutchinsky, S Endicott, C Realini, M Rechsteiner, and K G Standing, "The Proteasome Activator 11S REG or PA28: Recombinant REGa/REGb Hetero-oligomers are Heptamers", Biochemistry 38 , 5651 - 5658 (1999).

1998

M.E. McComb, A.N. Krutchinsky, W. Ens, K.G. Standing and H. Perreault, "Capillary Electrophoresis Time-of-Flight Mass Spectrometry for the Analysis of Biological Molecules", J. Chromatog. A, 800, 1-11 (1998)

W. Ens, K.G. Standing and I.V. Chernushevich (Eds.) "New Methods for the Study of Biomolecular Complexes ", NATO ASI Series, Kluwer, Dordrecht, NL, 1998, 354 pages

I.V. Chernushevich, W. Ens and K.G. Standing, "Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry", in ref. "W. Ens, K.G. Standing and I.V. Chernushevich (Eds.) "New Methods for the Study of Biomolecular Complexes", NATO ASI Series, Kluwer, Dordrecht, NL, 1998, 354 pages" on page 2, pp. 101-116.

A Ayed, A Krutchinsky, I Chernushevich, W Ens, HW Duckworth and K G Standing, "Observation of Noncovalent Complexes of Citrate Synthase and NADH by ESI/TOF Mass Spectrometry", in ref. "W. Ens, K.G. Standing and I.V. Chernushevich (Eds.) "New Methods for the Study of Biomolecular Complexes", NATO ASI Series, Kluwer, Dordrecht, NL, 1998, 354 pages" on page 2, pp135-139

S.G. Love, D.R. Engelbretsen, B. Garnham, A.C. Cavanagh, H. Morton, I.V. Chernushevich, K.G. Standing and P.F. Alewood, "Observation of Protein Heptamer Formation for Chemically Ligated Early Pregnancy Factor", in ref. "W. Ens, K.G. Standing and I.V. Chernushevich (Eds.) "New Methods for the Study of Biomolecular Complexes", NATO ASI Series, Kluwer, Dordrecht, NL, 1998, 354 pages" on page 2, pp. 171-179.

A. Ayed, A. Krutchinsky, W. Ens, K.G. Standing and H.W. Duckworth, "Quantitative Evaluation of Protein - Protein and Ligand - Protein Equilibria of a Large Allosteric Enzyme by Electrospray Ionization Time-of-Flight Mass Spectrometry", Rapid Commun. Mass Spectrom.,12, 339 - 344 (1998).

A.N. Krutchinsky, A.V. Loboda, V.L. Spicer, R. Dworschak, W. Ens and K.G. Standing, "Orthogonal Injection of MALDI Ions into a Time-of-Flight Spectrometer Through a Collisional Damping Interface", Rapid Commun. Mass Spectrom. , 12 , 508-518 (1998).

Q.P. Lei, X. Cui, D.M. Kurtz, Jr., I.J. Amster, I.V. Chernushevich and K.G. Standing, "Electrospray Mass Spectrometry Studies of Non-Heme Iron-Containing Proteins", Anal. Chem. 70 , 1838-1846 (1998).

D.L. Kirkpatrick, M. Kuperus, M. Dowdeswell, N. Potier, L.J. Donald, M. Kunkel, M. Berggren, M. Angulo and G. Powis, "Mechanisms of Inhibition of the Thioredoxin Growth Factor System by Antitumor 2-imidazolyl Disulfides", Biochem. Pharmacol ., 55 , 987-994 (1998).

N. Potier, L.J Donald, I. Chernushevich, A. Ayed, W. Ens, C.H. Arrowsmith, K.G. Standing and H.W. Duckworth, "Study of a Noncovalent trp Repressor-DNA Operator Complex by Electrospray Ionization Time-of-Flight Mass Spectrometry", Protein Science 7 (1998) 1388 - 1395.

A.N. Krutchinsky, IV Chernushevich, V Spicer, W Ens and K G Standing, "A Collisional Damping Interface for an Electrospray Ionization TOF Mass Spectrometer" J. Am. Soc. Mass Spectrom. 9 , 569-579 (1998).

R.G. Dworschak, W. Ens, K.G. Standing, K.R. Preston, B.A. Marchylo, M.J. Nightingale, S.G. Stevenson and D.W. Hatcher, "Analysis of Wheat Gluten Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry", J. Mass Spectrom. 33 , 429-435 (1998).

M.E McComb, R.D. Oleschuk, A. Chow, W. Ens, K.G. Standing, and H. Perreault, "Characterization of Hemoglobin Variants by MALDI-TOF MS Using a Polyurethane Membrane as the Sample Support", Anal. Chem. 70 , 5142-5149 (1998).

1997

I. V. Chernushevich, W. Ens and K.G. Standing, "Electrospray Ionization Time-of-Flight Mass Spectrometry", in Electrospray Ionization Mass Spectrometry , Ed R.B Cole, John Wiley & Sons, 1997, pp 203-234.

A. Shevchenko, I. Chernushevich, W. Ens, K.G. Standing, B. Thomson, M. Wilm and M. Mann, "Rapid de Novo Peptide Sequencing by a Combination of Nanoelectrospray, Isotopic Labeling and a Quadrupole/Time-of-flight Mass Spectrometer", Rapid Commun. Mass Spectrom.11, 1015-1024 (1997).

M.E. McComb, R.D. Oleschuk, D.M. Manley, L.Donald, A. Chow, J.D.J. ONeil, W. Ens, K.G. Standing and H. Perreault, "Use of a Non-Porous Polyurethane Membrane as a Sample Support for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Peptides and Proteins," Rapid Commun. Mass Spectrom., 11 , 1716-1722 (1997).

A.V. Tolmachev, I.V. Chernushevich, A.F. Dodonov and K.G. Standing, "A Collisional Focusing Ion Guide for Coupling an Atmospheric Pressure Ion Source to a Mass Spectrometer", Nucl. Instrum. and Methods B . 124, 112-119 (1997).

J. Bravo, I. Fita, J.C. Ferrer, W. Ens, A. Hillar, J. Switala and P.C. Loewen, "Identification of a Novel Bond between a Histidine and the Essential Tyrosine in Catalase HPII of Escherichia coli, Protein Science 6 , 1016-1023 (1997).

1996

K. Rose, W. Zeng, P-O. Raga Mey, I.V. Chernushevich, K.G. Standing and H.F. Gaertner, "Natural Peptides as Building Blocks for the Synthesis of Large Artificial Proteins with Hydrozone and Oxime Linkages", Bioconj. Chem ., 7 , 552-56 (1996).

G. Westmacott, W. Ens and K.G. Standing, "Secondary Ion and Electron Yield Measurements for Surfaces Bombarded by Large Molecular Ions", Nucl. Instrum. and Methods B , 108, 282-89 (1996).

I.V. Chernushevich. A.N. Verentchikov, W. Ens and K.G. Standing, "Effect of Ion-Molecule Collisions in the Vacuum Chamber of an Electrospray Time-of-Flight Mass Spectrometer on Mass Spectra of Proteins", J. Am. Soc. Mass Spectrom., 7 , 342-49 (1996).

L.J. Donald, I.V. Chernushevich, J. Zhou, A. Verentchikov, N. Poppe-Schriemer, D.J. Hosfield, J.B. Westmore, W. Ens, H.W. Duckworth and K.G. Standing, "Preparation and Properties of Pure, Full-Length IclR Protein of Escherichia coli . Use of Time-of-Flight Mass Spectrometry to Investigate the Problems Encountered", Protein Science , 5 , 1613-24 (1996).

M.C. Fitzgerald, I. Chernushevich, K.G. Standing, C.P. Whitman, and S.B.H. Kent, "Probing the Oligomeric Structure of an Enzyme by Electrospray Ionization Time-of-Flight Mass Spectrometry", Proc. Natl. Acad. Sci. (USA) , 93 , 6851-56 (1996).

1995

R.C. Werlen, M. Lankinen, A. Smith, I. Chernushevich, K.G. Standing, D.C. Blakey, H. Shuttleworth, R.G. Melton, R.E. Offord and K. Rose, "Site-Specific Immunoconjugates", Tumor Targeting , 1 , 251-58 (1995).

M.C. Fitzgerald, C.P. Whitman, I. Chernushevich, K.G. Standing and S.B.H. Kent, "Total Chemical Synthesis and Catalytic Properties of the Enzyme Enantiomers L- and D-4- Oxalocrotonate Tautomerase", J. Am. Chem. Soc. , 117 , 11075-80 (1995).

M.S. Sevinc, W. Ens and P.C. Loewen, "The Cysteines of Catalase HPII of Escherichia coli , including Cys438, which is Blocked, Do not Have a Catalytic Role", Eur. J. Biochem ., 230 , 127-132 (1995).

1994

A. N. Verentchikov, W. Ens and K. G. Standing, "Reflecting Time-of-Flight Mass Spectrometer with an Electrospray Ion Source and Orthogonal Extraction", Anal. Chem. 66 , 126-33 (1994).

X. J. Tang, C. F. Brewer, S. Saha, I. Chernushevich, W. Ens and K. G. Standing, "Investigation of Protein-Protein Noncovalent Interactions in Soybean Agglutinin by Electrospray Ionization Time-of-Flight Mass Spectrometry", Rapid Commun. Mass Spectrom. 8 , 750-754 (1994).